Journal: Frontiers in Bioengineering and Biotechnology

Article Title: An injectable curcumin-loaded hydrogel for neuroprotective treatment promote nerve tissue repair in rat severe spinal cord injury

doi: 10.3389/fbioe.2025.1655686

Figure Lengend Snippet: Evaluation of NF and GFAP in the spinal cord tissues and assessment of behavior function. (A) Representative fluorescent micrographs of immunofluorescence staining of NF (green) and GFAP (red) at Day 35 in spinal cord tissues of CBT-gel treatment and SCI group. Nuclei were stained by DAPI (Blue). (B–D) Tissues from the lesion epicenter (B) and adjacent (C) regions were quantified for positive areas of NF and GFAP, and the NF/GFAP ratios were calculated (D) . NF/GFAP ratio = (NF + area)/(GFAP + area in same region). (E) BBB scores of the animals during the 35-day recovery. Data are presented as mean and SD (n = 6–8). Statistical analysis was assessed by Mann-Whitney U test *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Following fixation in acetone at 4 °C, the sections were incubated overnight at 4 °C with specific primary antibodies targeting neurofilament (NF) (Proteintech, China), glial fibrillary acidic protein (GFAP) (Boster, China), et al. After washing, the sections were treated with secondary antibodies at 37 °C for 1 hour.

Techniques: Immunofluorescence, Staining, MANN-WHITNEY

Journal: Stem Cells Translational Medicine

Article Title: DLK1-expressing neural progenitor cells promote tissue repair and functional recovery after cervical spinal cord injury

doi: 10.1093/stcltm/szaf014

Figure Lengend Snippet: DLK1 expression modulates the in vivo differentiation of transplanted NPCs. (A) Representative immunohistochemical images of injured spinal cord sections transplanted with control NPCs or DLK1-NPC, stained for hNSE (human neuron-specific enolase, a neuronal marker), STEM123 (human GFAP, an astrocyte marker), and hGSTπ (GSTπ, an oligodendrocyte marker). Positive cells appear violet/purple as a result of the Vector VIP HRP substrate. Scale bar: 40 μm. (B) Violin plots showing the average number of hNSE⁺, STEM123⁺, and hGSTπ⁺ cells (cells/mm²) in the injured spinal cord from both NPC and DLK1-NPC groups. The central white line indicates the median, and the upper and lower white lines represent the first and third quartiles, respectively. Statistical analysis was performed using unpaired two-tailed t-tests (* P < .05).

Article Snippet: Additionally, the following human-specific primary antibodies were used: human neuron-specific enolase (hNSE), a marker for neurons (1:500; R&D Systems, MAP5169), human-specific GFAP (STEM123), a marker for astrocytes (1:500, Takara Bio.

Techniques: Expressing, In Vivo, Immunohistochemical staining, Control, Staining, Marker, Plasmid Preparation, Two Tailed Test